Article: Validation of Normalizations, Scaling, and Photofading Corrections for FRAP Data Analysis.
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Kang M; Andreani M; Kenworthy AK
PLoS ONE, 2015
10.1371/journal.pone.0127966.t002
Table 2
Diffusion FRAP models corrected for photofading and immobile fraction and their compatibilities.
In Binding Diffusion* (14), c k k th image, c k M c , β is the immobile fraction, and τ is the time needed for each image scan. Finally, g 1 and g 2 are the bleaching functions for bounded (c ) and free (u ) proteins.
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Underlying Kinetics Photofading Immobile Fraction FRAP data/FRAP equation Compatibility
Free Diffusion
Scaling of FRAP Equation
Linear Scaling
F D a t a ( t ) F i ⇐ Fit f d N D l M f ( t ) Eq 41 )Yes
Free Diffusion
Scaling of FRAP Equation
Affine Scaling
F D a t a ( t ) F i ⇐ Fit f d N D a M f ( t ) Eq 42 )No: Scaling order matters
Free Diffusion
Scaling of FRAP Data
Linear Scaling
F D a t a ( t ) F w h o l e ( t ) ⇐ Fit f N D l M f ( t ) Eq 38 )Yes
Free Diffusion
Scaling of FRAP Data
Affine Scaling
F D a t a ( t ) F w h o l e ( t ) ⇐ Fit f N D a M f ( t ) Eq 37 )Yes
Anomalous Diffusion
Scaling of FRAP Equation
Linear Scaling
F D a t a ( t ) F i ⇐ Fit f d A D l M f ( t ) Eq 41 )Yes
Anomalous Diffusion
Scaling of FRAP Equation
Affine Scaling
F D a t a ( t ) F i ⇐ Fit f d A D a M f ( t ) Eq 42 )No: Scaling order matters
Anomalous Diffusion
Scaling of FRAP Data
Linear Scaling
F D a t a ( t ) F w h o l e ( t ) ⇐ Fit f A D l M f ( t ) Eq 38 )Yes
Anomalous Diffusion
Scaling of FRAP Data
Affine Scaling
F D a t a ( t ) F w h o l e ( t ) ⇐ Fit f A D a M f ( t ) Eq 37 )Yes
Binding Diffusion
Scaling of FRAP Equation
Linear Scaling
F D a t a ( t ) F i ⇐ Fit c 2 M c 1 = δ ( 1 - β ) c k M c 1 = ( 1 - β ) ( g 2 k - 1 - [ g 2 k - 1 - c k - 1 M c 1 ( 1 - β ) ] e - τ k o f f ) g 1 Yes
Binding Diffusion
Scaling of FRAP Equation
Affine Scaling
NA
No: Regardless of Scaling Order
Inferred neuron-electrophysiology data values
Neuron Type
Neuron Description
Ephys Prop
Extracted Value
Standardized Value
Content Source
